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KMID : 0388220090160010023
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Journal of the Korean Rheumatism Association
2009 Volume.16 No. 1 p.23 ~ p.32
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The Marginal Zone B Cells have an Increased Antibody Expression in Mice with Collagen-induced Arthritis
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Woo Yun-Ju
Min Jun-Ki
Cho Mi-La
Kim Young-Joo
Jhung Joo-Yeon
Park Min-Jung
Moon Young-Mi
Park Mi-Kyung
Kim Ho-Youn
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Abstract
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Objectives:Mature B cells in the spleen of mouse can be divide into two main subsets: the follicular (FO) B cells and the marginal zone (MZ) B cells. In this study, we investigated which subtype of B cells is involved in the production of costimulatory molecules, cytokine and antibody during the induction of autoimmune arthritis.
Methods:The MZB and FOB cells isolated from DBA/1J induced- and collagen-induced arthritis (CIA) mice were stimulated with LPS or CpG. The costimulatory molecules were measured by flow cytometry (FACs). The cytokines were measured by ELISA. Production of antibodies by the MZB cells or FOB cells was measured by ELISA and the results were observed by confocal microscopy.
Results:The expression of co-stimulatory molecules was stronger in the MZB cells than that in the FOB cells. The production of cytokines (IL-10, IL-6) and antibodies was higher in the MZB cells. The IgG expression of the MZB cells, which is known to be associated with the acceleration of autoimmunity, was higher in the CIA mice than that in the DBA/1J mice.
Conclusion: We observed that the MZB cells were increased in the CIA mice. The costimulatory molecules, cytokine and auto-antibodies were increased in the MZB cells compared to that of the FOB cells. Our results suggest that MZB cells mainly produce autoantibodies, and they play a key role in development of autoimmune arthritis.
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KEYWORD
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MZB, FOB, Autoimmune arthritis, Autoantibody, IgG
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